Dataset Information

Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling.

ABSTRACT:

Objective

To investigate the immunomodulatory activity of polysaccharides from the roots of Brassica rapa.

Methods

The crude polysaccharide from roots of B. rapa (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis.

Results

Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide (NO), tumor necrosis factor α (TNFα) and upregulating mRNA levels of inducible NO synthase (iNOS) and TNFα. Furthermore, modulation of macrophage by BRP was indicated to be mediated via the activation of Akt and nuclear factor-kappa B (NF-κB).

Conclusion

The beneficial effects of BRP could be used as an immunotherapeutic adjuvant in treatment of inflammatory diseases.

SUBMITTER: Guo W

PROVIDER: S-EPMC9476711 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Immunomodulatory activity of polysaccharides from <i>Brassica rapa</i> by activating Akt/NF-κB signaling.

Guo Weiwei W Zhang Qianxiao Q Du Yu Y Guo Junting J Zhao Tingting T Bai Liping L An Xiqiang X

Chinese herbal medicines 20211007 1

<h4>Objective</h4>To investigate the immunomodulatory activity of polysaccharides from the roots of <i>Brassica rapa</i>.<h4>Methods</h4>The crude polysaccharide from roots of <i>B. rapa</i> (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis.<h4>Results</h4>Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide ...[more]

PMID: 36120126

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