Dataset Information
UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius.
Ontology highlight
ABSTRACT: Objective
In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from Carthamus tinctorius which contains diverse types of glycosides were mined. Methods: A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into Escherichia coli for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MSn. The structures of representative catalytic products were elucidated by MS and NMR.Results
UGT88B2 exhibited catalytic promiscuity and various patterns in glycosylation of flavonoids with high efficiency.Conclusion
A new glycosyltransferase named UGT88B2 was successfully mined and can be employed as enzymatic tools in glycosylation of flavonoids.
SUBMITTER: Sui SY
PROVIDER: S-EPMC9476720 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
UGT88B2: A promiscuous <i>O</i>-glycosyltransferase from <i>Carthamus tinctorius</i>.
Chinese herbal medicines 20200918 4
<h4>Objective</h4>In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from <i>Carthamus tinctorius</i> which contains diverse types of glycosides were mined. <i>Methods:</i> A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into <i>Escherichia coli</i> for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MS<sup>n</sup>. The structures of representativ ...[more]
