Distribution and diversity of endophytic fungi in Gentiana rigescens and cytotoxic activities.

ABSTRACT:

Objective

In the present study, Gentiana rigescens was screened for fungi communities to clarify their diversity and community assemblage in hosts. Meanwhile, the identification and activity assays of the strains were also conducted.

Methods

By culture-dependent (endophytic fungi isolations from plant sections) and culture-independent (metagenomic library and cloning from plant sections) techniques, fungi communities were studied. The metagenomic library was generated using direct DNA isolation of whole plants, plant radixes, plant stems, plant leaves, plant flowers and soils around the plant. Meanwhile, endophytes were isolated from all parts of G. rigescens plants. After fermentation of the fungi isolations, all the isolates were evaluated for their cytotoxic

SUBMITTER: Xu LL 

PROVIDER: S-EPMC9476734 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature