Two new terpenoids from Reduning Injection.

ABSTRACT:

Objective

To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning (RDN) Injection.

Methods

In this study, the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model. The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins, silica gel, ODS columns and reverse phase MPLC and HPLC repeatedly, and their structures were elucidated based on spectroscopic analysis (HR-ESI-MS, NMR, ECD) and chemical methods. Meanwhile, we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-α production in LPS stimulated RAW 264.7 macrophages.

Results

The 95% ethanol eluate of RDN Injection by the macroporous adsorption resi

SUBMITTER: Li HB 

PROVIDER: S-EPMC9476765 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature