Quantitative determination of multi-class bioactive constituents for quality control of Yiqi Jiangzhi Granules.

ABSTRACT:

Objective

To establish a reliable and sensitive method for evaluating quality of Yiqi Jiangzhi Granules (YQJZG).

Methods

Ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was employed for simultaneous determination of eight marker components. Separation was performed on an AQUITY UPLC® HSS T3 column, the mobile phase consisted of acetonitrile as the organic phase and 0.1% (volume percentage) formic acid as the aqueous. Eight marker components, ginsenoside Rg1 (GRg1), ginsenoside Re (GRe), ginsenoside Rb1 (Gb1), typhaneoside (TEO), isorhamnetin-3-O-neohespeidoside (IN), hesperidin (HPD), aurantio-obtusin-6-O-β-D-glucoside (AG) and curcumin (CCM), were detected by multiple reaction monitoring (

SUBMITTER: Guo S 

PROVIDER: S-EPMC9476781 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature