Differential dependency of human glioblastoma cells on vascular endothelial growth factor‑A signaling via neuropilin‑1.

ABSTRACT: Despite the high expression of neuropilin‑1 (NRP‑1) in human glioblastoma (GB), the understanding of its function as a co‑receptor of vascular endothelial growth factor receptors (VEGFRs) in angiogenesis is currently limited. Therefore, the aim of the present study was to elucidate the non‑classical function of NRP‑1 expression in human GB. Expression patterns of NRP‑1 and VEGF‑A were determined by sandwich ELISA, western blot analysis, or immunohistochemistry. Differential dependency of GB cells following ablation of VEGF‑A signaling was validated in vitro and in vivo. Cellular mechanism responsible for distinct response to VEGF‑A signaling was evaluated by western blotting and immunoprecipitation analysis. Prognostic implications were assessed using IHC analysis. GB cells e

SUBMITTER: Lee J 

PROVIDER: S-EPMC9477108 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature