Dataset Information

Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.

ABSTRACT: Gene expression is tightly regulated by RNA-binding proteins (RBPs) to facilitate cell survival, differentiation, and migration. Previous reports have shown the importance of the Insulin-like Growth Factor II mRNA-Binding Protein (IGF2BP1/IMP1/ZBP1) in regulating RNA fate, including localization, transport, and translation. Here, we generated and characterized a knockout mouse to study RBP regulation. We report that IGF2BP1 is essential for proper brain development and neonatal survival. Specifically, these mice display disorganization in the developing neocortex, and further investigation revealed a loss of cortical marginal cell density at E17.5. We also investigated migratory cell populations in the IGF2BP1[Formula: see text] mice, using BrdU labeling, and detected fewer mitotically active cells in the cortical plate. Since RNA localization is important for cellular migration and directionality, we investigated the regulation of β-actin messenger RNA (mRNA), a well-characterized target with established roles in cell motility and development. To aid in our understanding of RBP and target mRNA regulation, we generated mice with endogenously labeled β-actin mRNA (IGF2BP1[Formula: see text]; β-actin-MS2[Formula: see text]). Using endogenously labeled β-actin transcripts, we report IGF2BP1[Formula: see text] neurons have increased transcription rates and total β-actin protein content. In addition, we found decreased transport and anchoring in knockout neurons. Overall, we present an important model for understanding RBP regulation of target mRNA.

SUBMITTER: Nunez L

PROVIDER: S-EPMC9477413 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.

Núñez Leti L Buxbaum Adina R AR Katz Zachary B ZB Lopez-Jones Melissa M Nwokafor Chiso C Czaplinski Kevin K Pan Feng F Rosenberg Jason J Monday Hannah R HR Singer Robert H RH

Proceedings of the National Academy of Sciences of the United States of America 20220906 37

Gene expression is tightly regulated by RNA-binding proteins (RBPs) to facilitate cell survival, differentiation, and migration. Previous reports have shown the importance of the Insulin-like Growth Factor II mRNA-Binding Protein (IGF2BP1/IMP1/ZBP1) in regulating RNA fate, including localization, transport, and translation. Here, we generated and characterized a knockout mouse to study RBP regulation. We report that IGF2BP1 is essential for proper brain development and neonatal survival. Specifi ...[more]

PMID: 36067310

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Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.

Publications

Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.

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