A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes.

ABSTRACT: Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise. Automated patch-clamp (APC) approaches are more experimenter independent and offer high-throughput, but by design are predominantly limited to assays containing small, homogenous cells. In order to enable high-throughput APC assays on larger cells such as native cardiomyocytes isolated from mammalian hearts, we employed a fixed-well APC plate format. A broad range of detailed electrophysiological parameters including action potential, L-type calcium current and basal inward rectifier current were reliably acquired from isolated swine atrial and ventricular cardiomyocytes using APC. Effective pharmacological modulation also indicat

SUBMITTER: Seibertz F 

PROVIDER: S-EPMC9477872 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature