Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

ABSTRACT: As an important second messenger, calcium (Ca²⁺) regulates a wide variety of physiological processes. Disturbance of intracellular calcium homeostasis implicated in the occurrence of multiple types of diseases. Orai1 is the major player in mediating store-operated calcium entry (SOCE) and regulates calcium homeostasis in non-excitable cells. Over-expression and activation of Orai1 have been reported in breast cancer. However, its molecular mechanisms are still not very clear. Here, we demonstrated that Nucleolin (NCL) was a novel interacting partner of Orai1. NCL is a multifunctional nucleocytoplasmic protein and is upregulated in human breast tumors. The binding of C-termini of NCL (NCL-CT) to N-termini of Orai1 (Orai1-NT) is critical for mediating calcium influx and proliferat

SUBMITTER: Gu C 

PROVIDER: S-EPMC9478099 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature