Humanization and expression of IgG and IgM antibodies in plants as potential diagnostic reagents for Valley Fever.

ABSTRACT: Monoclonal antibodies (mAbs) are important proteins used in many life science applications, from diagnostics to therapeutics. High demand for mAbs for different applications urges the development of rapid and reliable recombinant production platforms. Plants provide a quick and inexpensive system for producing recombinant mAbs. Moreover, when paired with an established platform for mAb discovery, plants can easily be tailored to produce mAbs of different isotypes against the same target. Here, we demonstrate that a hybridoma-generated mouse mAb against chitinase 1 (CTS1), an antigen from Coccidioides spp., can be biologically engineered for use with serologic diagnostic test kits for coccidioidomycosis (Valley Fever) using plant expression. The original mouse IgG was modified and re

SUBMITTER: Jugler C 

PROVIDER: S-EPMC9478164 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature