Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer.

ABSTRACT:

Background

The peritoneal cavity contains many site-specific immune cells which constitute a unique immune microenvironment. However, it is unclear how the local immune signature is altered in patients with peritoneal metastases (PM).

Methods

Peritoneal lavage fluid or ascites were obtained from 122 patients with various stages of gastric cancer (GC). Cells recovered from peritoneal fluids were immunostained with mAbs for lymphocyte-, macrophage- and tumor cell-specific antigens and the frequencies of leukocyte subsets and antigen expression levels were evaluated with multi-color flowcytometry.

Results

The proportions of CD8(+) T cells, CD3(+)CD56(+) NKT-like cells, and CD3(-)CD56(+) NK cells to CD45(+) leukocytes were significantly reduced in patients with PM compare

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC9478385 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature