Histamine activates an intracellular Ca²⁺ signal in normal human lung fibroblast WI-38 cells.

ABSTRACT: Histamine is an inflammatory mediator that can be released from mast cells to induce airway remodeling and cause persistent airflow limitation in asthma. In addition to stimulating airway smooth muscle cell constriction and hyperplasia, histamine promotes pulmonary remodeling by inducing fibroblast proliferation, contraction, and migration. It has long been known that histamine receptor 1 (H1R) mediates the effects of histamine on human pulmonary fibroblasts through an increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i), but the underlying signaling mechanisms are still unknown. Herein, we exploited single-cell Ca²⁺ imaging to assess the signal transduction pathways whereby histamine generates intracellular Ca²⁺ signals in the human

SUBMITTER: Berra-Romani R 

PROVIDER: S-EPMC9478493 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature