Cooperative bundling by fascin generates actin structures with architectures that depend on filament length.

ABSTRACT: The assembly of actin-based structures with precisely defined architectures supports essential cellular functions, including motility, intracellular transport, and division. The geometric arrangements of the filaments within actin structures are stabilized via the association of crosslinking proteins, which bind two filaments simultaneously. Because actin polymerization and crosslinking occur concurrently within the dynamic environment of the cell, these processes likely play interdependent roles in shaping the architectures of actin-based structures. To dissect the contribution of polymerization to the construction of higher-order actin structures, we investigated how filament elongation affects the formation of simple, polarized actin bundles by the crosslinking protein fascin. Using pop

SUBMITTER: Sherer LA 

PROVIDER: S-EPMC9479110 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature