A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal modulation of bactericidal and anti-inflammation process in osteomyelitis treatment.

ABSTRACT:

Background

Drug resistance of pathogens and immunosuppression are the main causes of clinical stagnation of osteomyelitis. The ideal treatment strategy for osteomyelitis is to achieve both efficient antibacterial and bone healing through spatiotemporal modulation of immune microenvironment.

Methods

In this study, a bilayer hydrogel based on genetically engineered polypeptide AC₁₀A and AC₁₀ARGD was prepared by self-assembly. Ag₂S QDs@DSPE-mPEG₂₀₀₀-Ce6/Aptamer (AD-Ce6/Apt) was loaded in the top layer AC₁₀A hydrogel (AA) for antibacterial, and bone marrow-derived mesenchymal stem cells (BMSCs) were loaded in the lower layer AC₁₀ARGD hydrogel (MAR) for bone healing. The AD-Ce6/Apt can be released from the AA hydro

SUBMITTER: Xie X 

PROVIDER: S-EPMC9479290 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature