Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization.

ABSTRACT: Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence. We developed a photobleaching device using a light-emitting diode (LED) illuminator to quench autofluorescence in neural tissue. This device was equipped with 12 high-power LEDs (30 W per single LED) and an evaporative cooling system, and these features achieved highly efficient bleaching of autofluorescence and minimized tissue damage. Even after 60 min of photobleaching with evaporative cooling, the temperature gain of the tissue slide was suppressed almost completely. The autofluorescence of lipofuscin-like granules completely disappeared after 60 min of photobleaching, as did other bac

SUBMITTER: Tsuneoka Y 

PROVIDER: S-EPMC9479452 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature