Dataset Information

Neural activity patterns in the chemosensory network encoding vomeronasal and olfactory information in mice.

ABSTRACT: Rodents detect chemical information mainly through the olfactory and vomeronasal systems, which play complementary roles to orchestrate appropriate behavioral responses. To characterize the integration of chemosensory information, we have performed electrophysiological and c-Fos studies of the bulbo-amygdalar network in freely behaving female mice exploring neutral or conspecific stimuli. We hypothesize that processing conspecifics stimuli requires both chemosensory systems, and thus our results will show shared patterns of activity in olfactory and vomeronasal structures. Were the hypothesis not true, the activity of the vomeronasal structures would be independent of that of the main olfactory system. In the c-Fos analysis, we assessed the activation elicited by neutral olfactory or male stimuli in a broader network. Male urine induced a significantly higher activity in the vomeronasal system compared to that induced by a neutral odorant. Concerning the olfactory system, only the cortex-amygdala transition area showed significant activation. No differential c-Fos expression was found in the reward system and the basolateral amygdala. These functional patterns in the chemosensory circuitry reveal a strong top-down control of the amygdala over both olfactory bulbs, suggesting an active role of the amygdala in the integration of chemosensory information directing the activity of the bulbs during environmental exploration.

SUBMITTER: Pardo-Bellver C

PROVIDER: S-EPMC9479637 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Neural activity patterns in the chemosensory network encoding vomeronasal and olfactory information in mice.

Pardo-Bellver Cecília C Vila-Martin Manuel E ME Martínez-Bellver Sergio S Villafranca-Faus María M Teruel-Sanchis Anna A Savarelli-Balsamo Camila A CA Drabik Sylwia M SM Martínez-Ricós Joana J Cervera-Ferri Ana A Martínez-García Fernando F Lanuza Enrique E Teruel-Martí Vicent V

Frontiers in neuroanatomy 20220902

Rodents detect chemical information mainly through the olfactory and vomeronasal systems, which play complementary roles to orchestrate appropriate behavioral responses. To characterize the integration of chemosensory information, we have performed electrophysiological and c-Fos studies of the bulbo-amygdalar network in freely behaving female mice exploring neutral or conspecific stimuli. We hypothesize that processing conspecifics stimuli requires both chemosensory systems, and thus our results ...[more]

PMID: 36120099

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Project description:Motherhood entails changes in behavior with increased motivation for pups, induced in part by pregnancy hormones acting upon the brain. This work explores whether this alters sensory processing of pup-derived chemosignals. To do so, we analyse the expression of immediate early genes (IEGs) in the vomeronasal organ (VNO; Egr1) and centers of the olfactory and vomeronasal brain pathways (cFos) in virgin and late-pregnant females exposed to pups, as compared to buttons (socially neutral control). In pup-exposed females, we quantified diverse behaviors including pup retrieval, sniffing, pup-directed attack, nest building and time in nest or on nest, as well as time off nest. Pups induce Egr1 expression in the VNO of females, irrespective of their physiological condition, thus suggesting the existence of VNO-detected pup chemosignals. A similar situation is found in the accessory olfactory bulb (AOB) and posteromedial part of the medial bed nucleus of the stria terminalis (BSTMPM). By contrast, in the medial amygdala and posteromedial cortical amygdala (PMCo), responses to pups-vs-buttons are different in virgin and late-pregnant females, thus suggesting altered sensory processing during late pregnancy. The olfactory system also shows changes in sensory processing with pregnancy. In the main olfactory bulbs, as well as the anterior and posterior piriform cortex, buttons activate cFos expression in virgins more than in pregnant females. By contrast, in the anterior and especially posterior piriform cortex, pregnant females show more activation by pups than buttons. Correlation between IEGs expression and behavior suggests the existence of two vomeronasal subsystems: one associated to pup care (with PMCo as its main center) and another related to pup-directed aggression observed in some pregnant females (with the BSTMPM as the main nucleus). Our data also suggest a coactivation of the olfactory and vomeronasal systems during interaction with pups in pregnant females.

Project description:BackgroundThe chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues.ResultsWe have performed transcriptome analyses of the mouse vomeronasal organ, a sensory organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes.ConclusionsOur analyses provide little evidence supporting sexually dimorphic gene expression in the vomeronasal organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.

Dataset Information

Neural activity patterns in the chemosensory network encoding vomeronasal and olfactory information in mice.

Publications

Neural activity patterns in the chemosensory network encoding vomeronasal and olfactory information in mice.

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