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Altered composition of the oral microbiome in integrin beta 6-deficient mouse.

ABSTRACT: In periodontal disease (PD), bacterial biofilms suppress β6 integrin expression transforming growth factor-β1 signaling, resulting in gingival inflammation and bone loss. β6 integrin-null (Itgb6^-/- ) mice develop spontaneous PD. The aim of this study was to unravel potential differences in oral microbiome in wild-type (WT) and Itgb6^-/- FVB mice. Mouse oral microbiome was analyzed from 3- and 6-month-old WT and Itgb6^-/- . The periodontal inflammation and spontaneous bone loss were present in 3-month-old and advanced in 6-month-old Itgb6^-/- mice. The observed amplicon sequence variants (ASVs) of alpha diversity showed close similarity in 3-month-old and 6-month-old Itgb6^-/- mice. Chao1 and ACE methods revealed that the microbiome in Itgb6^-/- mice showed less diversity compared to the WT. UniFrac Principal Coordinate analyses (PCoA) showed a clear spatial segregation and clustering between Itgb6^-/- and WT mice in general, and between 3-month- and 6-month-old WT mice. Weighted PCoA showed the tight clustering and distinct separation of individual mouse samples within Itgb6^-/- and WT. The most abundant microbial classes varied between the sample groups. However, the genus Aggregatibacter significantly increased in the 6-month-old Itgb6^-/- mice. β6 integrin-deficient mice develop periodontal inflammation that may relate to dysbiosis in the microbiome that further promotes the disease process.

SUBMITTER: Uehara O

PROVIDER: S-EPMC9481083 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Altered composition of the oral microbiome in integrin beta 6-deficient mouse.

Uehara Osamu O Bi Jiarui J Zhuang Deshu D Koivisto Leeni L Abiko Yoshihiro Y Häkkinen Lari L Larjava Hannu H

Journal of oral microbiology 20220912 1

In periodontal disease (PD), bacterial biofilms suppress β6 integrin expression transforming growth factor-β1 signaling, resulting in gingival inflammation and bone loss. β6 integrin-null (Itgb6-/- ) mice develop spontaneous PD. The aim of this study was to unravel potential differences in oral microbiome in wild-type (WT) and Itgb6-/- FVB mice. Mouse oral microbiome was analyzed from 3- and 6-month-old WT and Itgb6-/- . The periodontal infla ...[more]

PMID: 36117552

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Altered composition of the oral microbiome in integrin beta 6-deficient mouse.

Publications

Altered composition of the oral microbiome in integrin beta 6-deficient mouse.

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