Customized strategies for high-yield purification of retinal pigment epithelial cells differentiated from different stem cell sources.

ABSTRACT: Retinal pigment epithelial (RPE) cell dysfunction and death are characteristics of age-related macular degeneration. A promising therapeutic option is RPE cell transplantation. Development of clinical grade stem-cell derived RPE requires efficient in vitro differentiation and purification methods. Enzymatic purification of RPE relies on the relative adherence of RPE and non-RPE cells to the culture plate. However, morphology and adherence of non-RPE cells differ for different stem cell sources. In cases whereby the non-RPE adhered as strongly as RPE cells to the culture plate, enzymatic method of purification is unsuitable. Thus, we hypothesized the need to customize purification strategies for RPE derived from different stem cell sources. We systematically compared five different RPE puri

SUBMITTER: Regha K 

PROVIDER: S-EPMC9481580 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature