Application of direct electrometry in studies of microbial rhodopsins reconstituted in proteoliposomes.

ABSTRACT: Microbial rhodopsins are the family of retinal-containing proteins that perform primarily the light-driven transmembrane ion transport and sensory functions. They are widely distributed in nature and can be used for optogenetic control of the cellular activities by light. Functioning of microbial rhodopsins results in generation of the transmembrane electric potential in response to a flash that can be measured by direct time-resolved electrometry. This method was developed by L. Drachev and his colleagues at the Belozersky Institute and successfully applied in the functional studies of microbial rhodopsins. First measurements were performed using bacteriorhodopsin from Halobacterium salinarum-the prototype member of the microbial retinal protein family. Later, direct electrometric

SUBMITTER: Siletsky SA 

PROVIDER: S-EPMC9481854 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature