Cas-CLOVER is a novel high-fidelity nuclease for safe and robust generation of T_SCM-enriched allogeneic CAR-T cells.

ABSTRACT: The use of T cells from healthy donors for allogeneic chimeric antigen receptor T (CAR-T) cell cancer therapy is attractive because healthy donor T cells can produce versatile off-the-shelf CAR-T treatments. To maximize safety and durability of allogeneic products, the endogenous T cell receptor and major histocompatibility complex class I molecules are often removed via knockout of T cell receptor beta constant (TRBC) (or T cell receptor alpha constant [TRAC]) and B2M, respectively. However, gene editing tools (e.g., CRISPR-Cas9) can display poor fidelity, which may result in dangerous off-target mutations. Additionally, many gene editing technologies require T cell activation, resulting in a low percentage of desirable stem cell memory T cells (T_SCM). We c

SUBMITTER: Madison BB 

PROVIDER: S-EPMC9481872 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature