Evaluation of the tert-butyl group as a probe for NMR studies of macromolecular complexes.

ABSTRACT: The development of methyl transverse relaxation optimized spectroscopy has greatly facilitated the study of macromolecular assemblies by solution NMR spectroscopy. However, limited sample solubility and stability has hindered application of this technique to ongoing studies of complexes formed on membranes by the neuronal SNAREs that mediate neurotransmitter release and synaptotagmin-1, the Ca²⁺ sensor that triggers release. Since the ¹H NMR signal of a ^tBu group attached to a large protein or complex can be observed with high sensitivity if the group retains high mobility, we have explored the use of this strategy to analyze presynaptic complexes involved in neurotransmitter release. For this purpose, we attached ^tBu groups at single cysteines o

SUBMITTER: Voleti R 

PROVIDER: S-EPMC9482097 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature