Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications.

ABSTRACT:

Background

Currently, there is no regenerative therapy for patients with neurological and neurodegenerative disorders. Cell-therapies have emerged as a potential treatment for numerous brain diseases. Despite recent advances in stem cell technology, major concerns have been raised regarding the feasibility and safety of cell therapies for clinical applications.

Methods

We generated good manufacturing practice (GMP)-compatible neural progenitor cells (NPCs) from transgene- and xeno-free induced pluripotent stem cells (iPSCs) that can be smoothly adapted for clinical applications. NPCs were characterized in vitro for their differentiation potential and in vivo after transplantation into wild type as well as genetically immunosuppressed mice.

Results

Generated NPCs had a

SUBMITTER: Rust R 

PROVIDER: S-EPMC9482172 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature