Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.

ABSTRACT:

Background

The analysis of chromatin integrity has become an important determinant of sperm quality. In frozen-thawed bovine sperm, neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood. The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics, and to assess if this parameter can predict pregnancy rates in cattle.

Results

A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods (0-2 h and 2-4 h), analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays, chromatin deprotamination and decondensation, sperm motility, viability, acrosomal status, and intracellular levels of total

SUBMITTER: Ribas-Maynou J 

PROVIDER: S-EPMC9482281 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature