Retinoic acid delays initial photoreceptor differentiation and results in a highly structured mature retinal organoid.

ABSTRACT:

Background

Human-induced pluripotent stem cell-derived retinal organoids are a valuable tool for disease modelling and therapeutic development. Many efforts have been made over the last decade to optimise protocols for the generation of organoids that correctly mimic the human retina. Most protocols use common media supplements; however, protocol-dependent variability impacts data interpretation. To date, the lack of a systematic comparison of a given protocol with or without supplements makes it difficult to determine how they influence the differentiation process and morphology of the retinal organoids.

Methods

A 2D-3D differentiation method was used to generate retinal organoids, which were cultured with or without the most commonly used media supplements, notably retinoi

SUBMITTER: Sanjurjo-Soriano C 

PROVIDER: S-EPMC9482314 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature