To deconvolve, or not to deconvolve: Inferences of neuronal activities using calcium imaging data.

ABSTRACT:

Background

With the increasing popularity of calcium imaging in neuroscience research, choosing the right methods to analyze calcium imaging data is critical to address various scientific questions. Unlike spike trains measured using electrodes, fluorescence intensity traces provide an indirect and noisy measurement of the underlying neuronal activities. The observed calcium traces are either analyzed directly or deconvolved to spike trains to infer neuronal activities. When both approaches are applicable, it is unclear whether deconvolving calcium traces is a necessary step.

Methods

In this article, we compare the performance of using calcium traces or their deconvolved spike trains for three common analyses: clustering, principal component analysis (PCA), and population de

SUBMITTER: Shen T 

PROVIDER: S-EPMC9482453 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature