Spatio-temporal analysis of prostate tumors in situ suggests pre-existence of treatment-resistant clones.

ABSTRACT: The molecular mechanisms underlying lethal castration-resistant prostate cancer remain poorly understood, with intratumoral heterogeneity a likely contributing factor. To examine the temporal aspects of resistance, we analyze tumor heterogeneity in needle biopsies collected before and after treatment with androgen deprivation therapy. By doing so, we are able to couple clinical responsiveness and morphological information such as Gleason score to transcriptome-wide data. Our data-driven analysis of transcriptomes identifies several distinct intratumoral cell populations, characterized by their unique gene expression profiles. Certain cell populations present before treatment exhibit gene expression profiles that match those of resistant tumor cell clusters, present after treatment. We conf

SUBMITTER: Marklund M 

PROVIDER: S-EPMC9482614 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature