Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPARγ during hyperlipidemia.

ABSTRACT: Valvular inflammation triggered by hyperlipidemia has been considered as an important initial process of aortic valve disease; however, cellular and molecular evidence remains unclear. Here, we assess the relationship between plasma lipids and valvular inflammation, and identify association of low-density lipoprotein with increased valvular lipid and macrophage accumulation. Single-cell RNA sequencing analysis reveals the cellular heterogeneity of leukocytes, valvular interstitial cells, and valvular endothelial cells, and their phenotypic changes during hyperlipidemia leading to recruitment of monocyte-derived MHC-II^hi macrophages. Interestingly, we find activated PPARγ pathway in Cd36⁺ valvular endothelial cells increased in hyperlipidemic mice, and the conservation

SUBMITTER: Lee SH 

PROVIDER: S-EPMC9482653 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature