CAV1 alleviated CaOx stones formation via suppressing autophagy-dependent ferroptosis.

ABSTRACT:

Background

Calcium oxalate (CaOx) is the most common type of kidney stone, but the mechanism of CaOx stones formation remains unclear. The injury of renal cells such as ferroptosis and autophagy has been considered a basis for stones formation.

Methods

We conducted transmission electron microscope (TEM), reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and C11-BODIPY analysis to explore whether CaOx could induce autophagy-dependent ferroptosis in vivo and in vitro. To explore the possible mechanism, we conducted bioinformatic analysis of patients with or without CaOx stones, Western blot and qPCR were used to identify the different genes we found in bioinformatic analysis.

Results

In our study, we found that CaOx could induce auto

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9482765 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature