MiR-378 associated with proliferation, migration and apoptosis properties in A549 cells and targeted NPNT in COPD.

ABSTRACT:

Background

microRNAs contribute to the development and progression of chronic obstructive pulmonary disease (COPD). However, the underlying molecular mechanisms are largely unclear. The goal of this study was to investigate the roles of miR-378 in alveolar epithelial type II cells and identify molecular mechanisms which contribute to the pathogenesis of COPD.

Materials and methods

Human alveolar epithelial (A549) cells were cultured in Dulbecco's Modified Eagle Medium. Cell proliferation was studied by using a cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis and cell cycle were analyzed by flow cytometry and wound healing and Transwell were used to analyze the cell migration and. We performed bioinformatics analysis including target gene prediction, ge

SUBMITTER: Qian G 

PROVIDER: S-EPMC9482771 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature