Towards a broader view of the metabolome: untargeted profiling of soluble and bound polyphenols in plants.

ABSTRACT: Phenylalanine (Phe) is a central precursor for numerous secondary plant metabolites with a multitude of biological functions. Recent studies on the fungal disease Fusarium head blight in wheat showed numerous Phe-derived defence metabolites to be induced in the presence of the pathogen. These studies also suggest a partial incorporation of Phe-derived secondary metabolites into the cell wall. To broaden the view of the metabolome to bound Phe derivatives, an existing approach using ¹³C-labelled Phe as tracer was extended. The developed workflow consists of three successive extractions with an acidified acetonitrile-methanol-water mixture to remove the soluble plant metabolites, followed by cell wall hydrolysis with 4M aqueous NaOH, acidification with aqueous HCl, and liquid-liqu

SUBMITTER: Doppler M 

PROVIDER: S-EPMC9482910 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature