Rapid and direct detection of hepatitis E virus in raw pork livers by recombinase polymerase amplification assays.

ABSTRACT: Hepatitis E virus (HEV) is a zoonotic pathogen that causes global hepatitis E. Outbreaks of hepatitis E are directly linked to the consumption of pork liver products. Herein reverse transcription recombinase polymerase amplification assays targeting the ORF2 gene were developed for the rapid detection of HEV by integrating the fluorescence detection platform (qRT-RPA) and the visible lateral flow biosensor by naked eyes (LFB RT-RPA). The qRT-RPA assay effectively detected HEV RNA with a limit of detection (LOD) of 154 copies/μl (95%CI: 126-333 copies/µl) in Genie III at 41°C for 20 min. Besides this, the LFB RT-RPA detected the HEV RNA with a LOD of 24 copies/μl (95%CI: 20-57 copies/µl) in an incubator block at 41°C for 20 min. The developed RT-RPA assays also showed good specificit

SUBMITTER: Wang K 

PROVIDER: S-EPMC9483107 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature