β subunit affects Na⁺ and K⁺ affinities of Na⁺/K⁺-ATPase: Na⁺ and K⁺ affinities of a hybrid Na⁺/K⁺-ATPase composed of insect α and mammalian β subunits.

ABSTRACT: The affinity for K⁺ of silkworm Na⁺/K⁺-ATPase, which is composed of α and β subunits, is remarkably lower than that of mammalian Na⁺/K⁺-ATPase, with a slightly higher affinity for Na⁺. Because the α subunit had more than 70% identity to the mammalian α subunit in the amino acid sequence, whereas the β subunit, a glycosylated protein, had less than 30% identity to the mammalian β subunit, it was suggested that the β subunit was involved in the affinities for Na⁺ and K⁺ of Na⁺/K⁺-ATPase. To confirm this hypothesis, we examined whether replacing the silkworm β subunit with the mammalian β subunit affected the affinities for Na⁺ and K⁺ of Na⁺/K⁺

SUBMITTER: Homareda H 

PROVIDER: S-EPMC9483571 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature