Scalable CRISPR-Cas9 chemical genetic screens in non-transformed human cells.

ABSTRACT: Pooled lentiviral CRISPR-Cas9 screens are utilized for assessing the differential sensitivity or resistance of many single-gene knockouts to a compound. Here, we present a scalable approach for high-throughput compound screening by utilizing a small custom library. We describe steps to perform a proof-of-principle chemical screen in non-transformed hTERT RPE-1 TP53^-/- cells with higher coverage and greater timepoint resolution compared to genome-wide screens. This approach can be adapted for use in various cell lines, compounds, and other focused sgRNA libraries.

SUBMITTER: Lin K 

PROVIDER: S-EPMC9483651 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature