Immune features of the peritumoral stroma in pancreatic ductal adenocarcinoma.

ABSTRACT:

Background

The peritumoral stroma is a hallmark of pancreatic ductal adenocarcinoma (PDA) with implications for disease development, progression and therapy resistance. We systematically investigated immune features of the stroma in PDA patients to identify markers of clinical importance and potential therapeutic targets.

Methods

Tissue and blood samples of 51 PDA patients with clinical and follow-up information were included. Laser Capture Microdissection allowed us to analyze the stromal compartment in particular. Systematic immunohistochemistry, followed by software-based image analysis were conducted. Also, multiplex cytokine analyses (including 50 immune-related molecules) were performed. Functional analyses were performed using patient-derived 3D bioprints. Clinical in

SUBMITTER: Ahmed A 

PROVIDER: S-EPMC9483939 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature