Dataset Information

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment.

ABSTRACT: The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale (e.g., by force spectroscopy with functionalised probes). Our data demonstrates that the glycocalyx configuration affects spatio-temporal nanotopography-sensitive mechanotransductive events at the cell/microenvironment interface. Opposing effects of major glycocalyx removal were observed, when comparing flat and specific nanotopographical conditions. The excessive retrograde actin flow speed and force loading are strongly reduced on certain nanotopographies upon strong reduction of the native glycocalyx, while on the flat substrate we observe the opposite trend. Our results highlight the importance of the glycocalyx configuration in a molecular clutch force loading-dependent cellular mechanism for mechanosensing of microenvironmental nanotopographical features.

SUBMITTER: Chighizola M

PROVIDER: S-EPMC9484177 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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The glycocalyx affects the mechanotransductive perception of the topographical microenvironment.

Chighizola Matteo M Dini Tania T Marcotti Stefania S D'Urso Mirko M Piazzoni Claudio C Borghi Francesca F Previdi Anita A Ceriani Laura L Folliero Claudia C Stramer Brian B Lenardi Cristina C Milani Paolo P Podestà Alessandro A Schulte Carsten C

Journal of nanobiotechnology 20220919 1

The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale (e.g., by force spectroscopy with functionalised pr ...[more]

PMID: 36123687

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Project description:Understanding the factors that affect human thermal responses is necessary to properly design and operate low-energy buildings. It has been suggested that factors not related to the thermal environment can affect thermal responses of occupants, but these factors have not been integrated in thermal comfort models due to a lack of knowledge of indoor factor interactions. While some studies have investigated the effect of electric light on thermal responses, no study exists on the effect of daylight. This study presents the first controlled experimental investigation on the effect of daylight quantity on thermal responses, combining three levels of daylight illuminance (low ~130 lx, medium ~600 lx, and high ~1400 lx) with three temperature levels (19, 23, 27 °C). Subjective and objective thermal responses of 84 participants were collected through subjective ratings on thermal perception and physiological measurements, respectively. Results indicate that the quantity of daylight influences the thermal perception of people specifically resulting in a cross-modal effect, with a low daylight illuminance leading to a less comfortable and less acceptable thermal environment in cold conditions and to a more comfortable one in warm conditions. No effect on their physiological responses was observed. Moreover, it is hypothesised that a warm thermal environment could be tolerated more whenever daylight is present in the room, as compared to the same thermal condition in a room lit with electric lights. Findings further the understanding of factors affecting human thermal responses and thermal adaptation processes in indoor environments and are relevant for both research and practice. The findings suggest that daylight should be considered as a factor in thermal comfort models and in all thermal comfort investigations, as well as that thermal and daylight illuminance conditions should be tuned and changed through the operation and design strategy of the building to guarantee its occupants' thermal comfort in existing and future structures.

Dataset Information

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment.

Publications

The glycocalyx affects the mechanotransductive perception of the topographical microenvironment.

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