Transcription factor AP2 enhances malignancy of non-small cell lung cancer through upregulation of USP22 gene expression.

ABSTRACT:

Background

Ubiquitin-specific protease 22 (USP22), a putative cancer stem cell marker, is frequently upregulated in cancers, and USP22 overexpression is associated with aggressive growth, metastasis, and therapy resistance in various human cancers including lung cancer. However, USP22 gene amplification seldom occurs, and the mechanism underlying USP22 upregulation in human cancers remains largely unknown.

Methods

A luciferase reporter driven by a promoter region of USP22 gene was selectively constructed to screen against a customized siRNA library targeting 89 selected transcription factors to identify potential transcription factors (TFs) that regulate USP22 expression in human non-small cell lung cancers (NSCLC). Association of identified TFs with USP22 and potential role

SUBMITTER: Sun T 

PROVIDER: S-EPMC9484186 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature