Effects of Coronary Artery Disease-Associated Variants on Vascular Smooth Muscle Cells.

ABSTRACT:

Background

Genome-wide association studies have identified many genetic loci that are robustly associated with coronary artery disease (CAD). However, the underlying biological mechanisms are still unknown for most of these loci, hindering the progress to medical translation. Evidence suggests that the genetic influence on CAD susceptibility may act partly through vascular smooth muscle cells (VSMCs).

Methods

We undertook genotyping, RNA sequencing, and cell behavior assays on a large bank of VSMCs (n>1499). Expression quantitative trait locus and splicing quantitative trait locus analyses were performed to identify genes with an expression that was influenced by CAD-associated variants. To identify candidate causal genes for CAD, we ascertained colocalizations of VSMC expre

SUBMITTER: Solomon CU 

PROVIDER: S-EPMC9484647 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature