Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer.

ABSTRACT:

Background

Cisplatin resistance is observed in patients with laryngeal cancer. The present study was designed to explore the efficacy of oxaliplatin on laryngeal cancer and elucidate the underlying mechanisms.

Methods

Cell viability was determined by using MTT assays. Cell apoptosis was determined by using annexin V and propidium iodide (PI) staining. Flow cytometry and immunofluorescence were applied to determine the levels of calreticulin (CALR) and DiD (1,1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine). Flow cytometry was applied to analyze the levels of CD83, CD86, IFN-γ-producing CD8⁺ T cells, and CD4⁺CD25⁺FoxP3⁺ Tregs. The levels of adenosine triphosphate (ATP) were determined by using a chemiluminescent ATP kit a

SUBMITTER: Wang J 

PROVIDER: S-EPMC9484908 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature