A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing.

ABSTRACT: Human pluripotent stem cells (hPSCs) are an exciting and promising source to enable cell replacement therapies for a variety of unmet medical needs. Though hPSCs can be successfully derived into numerous physiologically relevant cell types, effective translation to the clinic is limited by challenges in scalable production of high-quality cells, cellular immaturity following the differentiation process, and the use of animal-derived components in culture. To address these limitations, we have developed a fully defined, reproducible, and tunable thermoreversible polymer for high-quality, scalable 3D cell production. Our reproducible synthesis method enables precise control of gelation temperature (24°C-32°C), hydrogel stiffness (100-4000 Pa), and the prevention of any unintended covalent cr

SUBMITTER: Johnson HJ 

PROVIDER: S-EPMC9485071 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature