A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production.

ABSTRACT: Recent findings suggest that the ribosome itself modulates gene expression. However, whether ribosomes change composition across cell types or control cell fate remains unknown. Here, employing quantitative mass spectrometry during human embryonic stem cell differentiation, we identify dozens of ribosome composition changes underlying cell fate specification. We observe upregulation of RPL10A/uL1-containing ribosomes in the primitive streak followed by progressive decreases during mesoderm differentiation. An Rpl10a loss-of-function allele in mice causes striking early mesodermal phenotypes, including posterior trunk truncations, and inhibits paraxial mesoderm production in culture. Ribosome profiling in Rpl10a loss-of-function mice reveals decreased translation of mesoderm regulators, inc

SUBMITTER: Genuth NR 

PROVIDER: S-EPMC9485161 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature