Potential regulatory role of miRNA and mRNA link to metabolism affected by chronic intermittent hypoxia.

ABSTRACT: Aim: Intermittent hypoxia (IH) is the prominent feature of obstructive sleep apnea (OSA) pathophysiology, which is an in dependent risk factor of cardiovascular complications. The effects of IH on adipocyte metabolism were explored by high-throughput sequencing technology. Methods: Plasma was collected from OSA patients and control group to perform mRNA sequencing. 3T3-L1 cells were differentiated into adipocytes then subjected to a 5%-21% O₂ hypoxic environment (IH) for 24 h. High-throughput sequencing method was used to determine differential mRNA and miRNA patterns in fat cells exposed to IH. We then performed Gene Ontology (GO) analysis, identified relevant KEGG pathways and miRNA-target-pathways. Results: Sequencing data showed that OSA affected the exp

SUBMITTER: Duan Y 

PROVIDER: S-EPMC9485438 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature