Production of recombinant human xCT (SLC7A11) and reconstitution in proteoliposomes for functional studies.

ABSTRACT: The plasma membrane transporter xCT belongs to the SLC7 family and has the physiological role of mediating the exchange of glutamate and cystine across the cell plasma membrane, being crucial for redox control. The xCT protein forms a heterodimer with the ancillary protein CD98. Over the years, xCT became a hot pharmacological target due to the documented over-expression in virtually all human cancers, which rely on cystine availability for their progression. Notwithstanding, several unknown aspects of xCT biology still exist that require a suitable single protein experimental model, to be addressed. To this aim, the recombinant host Escherichia coli has been exploited to over-express the human isoform of xCT. In this widely used and low-cost system, the optimization for growth and

SUBMITTER: Galluccio M 

PROVIDER: S-EPMC9485625 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature