Quantitative Proteomics Identifies Proteins Enriched in Large and Small Extracellular Vesicles.

ABSTRACT: There is a long-held consensus that several proteins are unique to small extracellular vesicles (EVs), such as exosomes. However, recent studies have shown that several of these markers can also be present in other subpopulations of EVs to a similar degree. Furthermore, few markers have been identified as enriched or uniquely present in larger EVs, such as microvesicles. The aim of this study was to address these issues by conducting an in-depth comparison of the proteome of large and small EVs. Large (16,500g) and small EVs (118,000g) were isolated from three cell lines using a combination of differential ultracentrifugation and a density cushion and quantitative mass spectrometry (tandem mass tag-liquid chromatography-tandem mass spectrometry) was used to identify differently enriched pr

SUBMITTER: Lischnig A 

PROVIDER: S-EPMC9486130 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature