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Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7.

ABSTRACT: Aronia melanocarpa anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, the antibacterial mechanism of AMAs against Escherichia coli O157: H7 remains unclear. In this study, the tandem mass tag (TMT) quantitative proteomics was used to elucidate the potential antibacterial mechanisms of AMAs against E. coli O157: H7 cells. Ultrastructural observation and reactive oxygen species (ROS) levels detection showed that AMAs destroyed the integrity of E. coli O157: H7 cell membrane, led to the aggregation of contents and caused the increase of intracellular ROS level. TMT-based proteomic analysis suggested that AMAs can enter cells through mechanosensitive channels and inhibit the activity of heat shock proteins; disturb the metabolism of carbohydrates, amino acids and lipids in cells. The results of this study provide a reference for the further development of plant-derived antimicrobial agents.

SUBMITTER: Deng H 

PROVIDER: S-EPMC9486179 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Proteomic analyses revealed the antibacterial mechanism of <i>Aronia melanocarpa</i> isolated anthocyanins against <i>Escherichia coli</i> O157: H7.

Deng Haotian H   Kong Yanwen Y   Zhu Jinyan J   Jiao Xinyao X   Tong Yuqi Y   Wan Meizhi M   Zhao Yang Y   Lin Sixu S   Ma Yan Y   Meng Xianjun X  

Current research in food science 20220915

<i>Aronia melanocarpa</i> anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, the antibacterial mechanism of AMAs against <i>Escherichia coli</i> O157: H7 remains unclear. In this study, the tandem mass tag (TMT) quantitative proteomics was used to elucidate the potential antibacterial mechanisms of AMAs against <i>E. coli</i> O157: H7 cells. Ultrastructural observation and reactive oxygen species (ROS)  ...[more]

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