The cyclin dependent kinase inhibitor p21^Cip1/Waf1 is a therapeutic target in high-risk neuroblastoma.

ABSTRACT: Platinum-based chemotherapies such as cisplatin are used as first-line treatment for the paediatric tumour neuroblastoma. Although the majority of neuroblastoma tumours respond to therapy, there is a high fraction of high-risk neuroblastoma patients that eventually relapse with increased resistance. Here, we show that one key determinant of cisplatin sensitivity is phosphorylation of the cyclin-dependent kinase inhibitor p21^Cip1/Waf1. A panel of eight neuroblastoma cell lines and a TH-MYCN mouse model were investigated for the expression of p21^Cip1/Waf1 using RT-qPCR, Western blot, and immunofluorescence. This was followed by investigation of sensitivity towards cisplatin and the p21^Cip1/Waf1 inhibitor UC2288. Whereas the cell lines and the mouse mod

SUBMITTER: Sorteberg AL 

PROVIDER: S-EPMC9486206 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature