Diversity of reptile sex chromosome evolution revealed by cytogenetic and linked-read sequencing.

ABSTRACT: Reptile sex determination is attracting much attention because the great diversity of sex-determination and dosage compensation mechanisms permits us to approach fundamental questions about mechanisms of sex chromosome turnover. Recent studies have made significant progress in better understanding diversity and conservation of reptile sex chromosomes, with however no reptile master sex determination genes identified. Here we describe an integrated genomics and cytogenetics pipeline, combining probes generated from the microdissected sex chromosomes with transcriptome and genome sequencing to explore the sex chromosome diversity in non-model Australian reptiles. We tested our pipeline on a turtle, two species of geckos, and a monitor lizard. Genes identified on sex chromosomes were compared

SUBMITTER: Zhu ZX 

PROVIDER: S-EPMC9486513 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature