MsPIPE: a pipeline for the analysis and visualization of whole-genome bisulfite sequencing data.

ABSTRACT:

Background

DNA methylation is an important epigenetic modification that is known to regulate gene expression. Whole-genome bisulfite sequencing (WGBS) is a powerful method for studying cytosine methylation in a whole genome. However, it is difficult to obtain methylation profiles using the WGBS raw reads and is necessary to be proficient in all types of bioinformatic tools for the study of DNA methylation. In addition, recent end-to-end pipelines for DNA methylation analyses are not sufficient for addressing those difficulties.

Results

Here we present msPIPE, a pipeline for DNA methylation analyses with WGBS data seamlessly connecting all the required tasks ranging from data pre-processing to multiple downstream DNA methylation analyses. The msPIPE can generate various methy

SUBMITTER: Kim H 

PROVIDER: S-EPMC9487059 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature