ANGPTL3 is involved in kidney injury in high-fat diet-fed mice by suppressing ACTN4 expression.

ABSTRACT:

Objective

We wanted to explore how angiopoietin-like 3 (ANGPTL3) impact hyperlipidemia-induced renal injury.

Methods

ANGPTL3 knockout mice and wild-type C57 mice were set up in four groups (N = 5) depending on a normal or 60% high-fat diet: wild-type with normal diet (WT), angptl3-/- with normal diet (KO), wild-type + high-fat diet (WT + HF) and angptl3-/- + high-fat diet (KO + HF). The detection time points were the 9th, 13th, 17th and 21st weeks after modeling. Serum lipid and urinary protein levels of mice in each group were detected, and pathological changes in the kidney were analyzed. Moreover, the expression of ANGPTL3, α-actinin-4 (ACTN4), CD2-associated protein (CD2AP) and podocin was tested in the glomerulus by immunohistochemistry (IHC).

Results

In the WT +

SUBMITTER: Li G 

PROVIDER: S-EPMC9487085 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature