Effect of Recombinant Human Lubricin on Model Tear Film Stability.

ABSTRACT:

Purpose

To investigate and quantify the effect of recombinant human lubricin (rh-lubricin) on model tear film stability.

Methods

A custom-built, interferometry-based instrument called the Interfacial Dewetting and Drainage Optical Platform was used to create and record the spatiotemporal evolution of model acellular tear films. Image segmentation and analysis was performed in MATLAB to extract the most essential features from the wet area fraction versus time curve, namely the evaporative break-up time and the final wet area fraction (A10). These two parameters indicate the tear film stability in the presence of rh-lubricin in its unstressed and stressed forms.

Results

Our parameters successfully captured the trend of increasing tear film stability with increasing rh-

SUBMITTER: Cui KW 

PROVIDER: S-EPMC9487168 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature