Identification of the Shigella flexneri Wzy Domain Modulating Wzz_pHS-2 Interaction and Detection of the Wzy/Wzz/Oag Complex.

ABSTRACT: Shigella flexneri implements the Wzy-dependent pathway to biosynthesize the O antigen (Oag) component of its surface lipopolysaccharide. The inner membrane polymerase Wzy_SF catalyzes the repeat addition of undecaprenol-diphosphate-linked Oag (Und-PP-RUs) to produce a polysaccharide, the length of which is tightly regulated by two competing copolymerase proteins, Wzz_SF (short-type Oag; 10 to 17 RUs) and Wzz_pHS-2 (very-long-type Oag; >90 RUs). The nature of the interaction between Wzy_SF and Wzz_SF/Wzz_pHS-2 in Oag polymerization remains poorly characterized, with the majority of the literature characterizing the individual protein constituents of the Wzy-dependent pathway. Here, we report instead a major investigation into the s

SUBMITTER: Ascari A 

PROVIDER: S-EPMC9487639 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature